HomeYou must come to lab prepared--this requires you to read the experimental protocols, not just print a copy of them. Bring whatever information you need to perform the experiments. The procedures for each day are available here and you will be given any additional information in the pre-lab lectures.
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Pre-Lab |
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Experimental Procedures |
Wiring parts to function collectively |
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plasmid DNA mini prep |
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restriction enzyme (RE) digests | |
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phosphatase treatment of vector |
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Pre-Lab |
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Experimental Procedures |
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A genetic switch |
agarose gel analysis of digests | |
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gel purification of insert and vector DNA | |
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DNA ligation | ||
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bacterial transformation and selection |
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Pre-Lab |
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Experimental Procedures |
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Matt Bennett (Invited Speaker) |
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PCR colony screen |
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PCR amplification of fragments for assembly | |
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preparation of vector for assembly |
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Pre-Lab |
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Experimental Procedures |
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Christian Ray (Invited Speaker) |
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agarose gel analysis of PCR: size & yield |
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purification of DNA fragments for assembly | |
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isothermal assembly of vectors |
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Pre-Lab |
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Experimental Procedures |
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agarose gel analysis of assembly reaction | |
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Promoter regulation |
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transformation with assembled vector |
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fluorescent plate reader training |
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Pre-Lab |
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Experimental Procedures |
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fluorescence analysis of pTetR-GFP | |
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transformation with two plasmids | |
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exp. design: choose REs to confirm ITA tsfmn. (Homework) |
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Pre-Lab |
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Experimental Procedures |
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mini prep/RE digests to confirm ITA plasmid | |
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exp. design: growth conditions to test circuit (in-lab) |
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Pre-Lab |
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Experimental Procedures |
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fluorescence analysis of pVenus-Grx/pTara |
We would like to thank New England Biolabs for their generous support of this laboratory course
Copyright, Acknowledgements,
and Intended Use
Created by B. Beason (bbeason@rice.edu),
Rice University, 4 January 2008
Updated 3 November 2011