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Destiny is no matter of chance. It is a matter of choice.
It is not a thing to be waited for. It is a thing to be achieved.
William Jennings Bryan
Day 8: Functional analysis of complex circuit
Assignments Due
- Go to the Presentation
Schedule
- Presenters see the guidelines for Journal
Club Presentation
- Everyone else
read listed articles and
be prepared for discussion
- Articles in OWL-Space resources
- In Vivo Fluorescent Detection of Fe-S Clusters Coordinated
by Human GRX2 (Chem. & Biol. 16: 1299-1309, 2009)
- Genetically Encoded Sensors to Elucidate Spatial Distribution of Cellular Zinc (J. Biol. Chem. 284: 16289-16297, 2009)
Overview of Experiment
Today you study regulation of pET-Venus-GRX2+pTara
system (in DH5alpha).
Experimental goal: How do you tune the output of the
logic gate?
What happens if glucose is present?
What does the transfer curve look like if we vary the concentration of arabinose and keep IPTG constant?
What does the transfer curve look like if we vary the concentration of IPTG and keep arabinose constant?
Copyright, Acknowledgements,
and Intended Use
Created by B. Beason (bbeason@rice.edu),
Rice University, 21 November 2007
Updated 2 November 2011