You must come to lab prepared--this requires you to READ the experimental protocols on the course web site BEFORE coming to lab, not just print a copy of them and bring it with you. Bring only the information you need to perform the experiments. The procedures for each day are available here and you will be given any additional information in the pre-lab lectures.
SPECIAL NOTE: Record enough procedure details in your notebook during each day of lab so that you can repeat these procedures using your notebook as the MAIN resource. An exception to this note are the instructions for using the spectrophotometers; there are 3 models, and since you may not always use the same type, you can have those instructions handy in your "BIOC 311 Binder" (for loose data).In this laboratory course, we use several pieces of equipment and many different reagents from Bio-Rad. Check out their web page for useful information on life science research products. On certain lab days you may want to read specific Bio-Rad manuals.
Preparation |
|
Experimental Procedures |
Honor Code Policy |
|
SEC column preparation |
Team library project |
|
Cell lysis & tissue homogenization |
Enzyme stability and assays |
|
Bradford assay |
Protein extraction |
|
ADA assay |
Bradford assay |
|
Ammonium sulfate precipitation |
Salt precipitation |
|
Preparation |
|
Experimental Procedures |
Research Paper overview |
|
Size exclusion chromatography |
Plagiarism |
|
Qualitative ADA activity assays |
Size exclusion chromatography theory |
|
Measurement at 280 nm |
Detection of ADA Activity |
|
Selection/preparation of anion exchange buffer |
Buffers for ion exchange |
|
Dialysis |
Dialysis |
|
Preparation |
|
Experimental Procedures |
Writing the Materials and Methods |
|
Isoelectric focusing (IEF) |
Isoelectric focusing |
|
ADA activity stain of IEF gel |
Coupled enzyme assays |
|
Silver stain of IEF gel |
Preparation |
|
Experimental Procedures |
Writing the Introduction |
|
Anion exchange chromatography |
Anion exchange chromatography set-up |
|
Qualitative and quantitative ADA activity assays |
Protein determination |
|
Measurement at 280 nm |
Protein purification tables |
|
Desalting Q fraction |
Preparation |
|
Experimental Procedures |
Writing the Results |
|
SDS-PAGE |
Electrophoresis applications |
|
Silver stain of SDS-PAGE gel |
Western blot development |
|
Electrophoretic transfer |
|
Western blot development |
Preparation |
|
Experimental Procedures |
Writing the Discussion and Abstract |
|
Kinetic studies |
Spectrophotometry |
|
|
Kinetic review |
|
Copyright, Acknowledgements,
and Intended Use
Created by B. Beason (bbeason@rice.edu),
Rice University, 25 February 2010
Updated 6 April 2016