BIOC 311 Team Library Project

The details for this project are in the "Library Project" Assignment in Canvas. The class will collect materials to be used as references for the course. As you gather your information try to evaluate the source materials for ease of use, for amount of information, and for the type of information. Note any problems that you encounter.
Examples of ACCEPTABLE references include

Examples of UNACCEPTABLE references include NOTE: DO NOT LIMIT YOUR LITERATURE SEARCH TO RECENT PUBLICATIONS ONLY--some of the references needed were published in 1940's to 1980's. ONLINE references are acceptable if you include all the information listed below and an online ID.

Assignment due on Day 2 or 3


Our lab has obtained a strain of E. coli that is reportedly transformed with a plasmid to express murine adenosine deaminase (ADA). Your assignment is to characterize the deaminase protein produced in these cells to compare the findings with the native deaminase extracted from mouse tissue.

Before beginning the project the following information is required:

Background information:

What reaction does adenosine deaminase catalyze?
What are the physiological functions associated with this reaction?
What mammalian tissues contain adenosine deaminase? What about non-mammals?
What problems result if ADA is not present? What if ADA is present in excess?

Known physical characteristics of adenosine deaminase (especially from mouse and E. coli):

molecular mass
isoelectric point (pI)
Km's for adenosine and 2'-deoxyadenosine
Ki's and type of inhibition of common inhibitors (especially N6-methyladenosine or methylaminopurine)

Assay for adenosine deaminase (report at least two different methods; one of these must be a spectrophotometric assay):

reaction - basis of the assay (what is being monitored?)
buffer conditions
substrate concentrations
spectrophotometric assay

Purification schemes and conditions that have been used for:

E. coli adenosine deaminase

mouse adenosine deaminase

Copyright, Acknowledgements, and Intended Use
Created by B. Beason (bbeason@rice.edu), Rice University, 11 June 1999
Updated 22 August 2016