Home
Experimental Molecular Biology Assignments and Grading
BIOS 413 is a CAPSTONE course: the crowning
achievement, the acme of your undergraduate laboratory training
at Rice. We assess your abilities to apply knowledge you've
gained in other laboratory and lecture courses to the presentation
and communication of a real research project.
SPECIAL NOTE: Bios 413 requires greater independence
than Bios 311 or Bios 313. The experimental procedures are
often less demanding than those you performed in Bios 311;
HOWEVER, just because the experiments may be more "cookbook" does
not mean the lab requires less effort. I place a greater
emphasis on analysis, interpretation, and presentation of
data; if you keep a "sloppy" notebook
and slap a poster together at the last minute without really
understanding "why" you did a procedure and "what" the
results mean, you will be disappointed with your final grade.
Several of you have worked or are working in Dr. Braam's lab.
Although this experience may give you an advantage in performing
the experiments and/or understanding the results, you are
still expected to meet our standards (see our Laboratory
Learning Objectives) for keeping a laboratory
notebook and preparing a scientific poster. Do not make the
mistake of assuming this lab will be "easy" just
because you've worked with Arabidopsis before. Remember,
the challenge with molecular biology is figuring out what the
results really mean, especially when those results are unexpected!
Remember, the
undergraduate program is a series of steps. Keep in mind that
a level of performance that would result in a B/B+ or sometimes
even an A at the introductory level, does not (and should not)
translate into a high grade at the advanced level.
We forgive a lot of mistakes early on but you must recall the
lessons learned from these mistakes when you conduct similar
work at an advanced level.
Additionally, the criteria we use to evaluate your performance in an advanced laboratory course are different from those we use in an introductory course.
"Fairness" in this course means everyone is evaluated on the same criteria.
Since everyone does not have the same ability, everyone does not get the same grade; this outcome is not "unfair" but rather is simply reality.
- Please read my Honor Code Policy.
- A total of 200 POINTS is available in this class.
Due dates are subject to change. Do not ask
for an individual extension to a due date because you
have a paper due, or three exams, or you lost your disk; due
dates are set so that you have plenty of time to complete your
work. Don't wait until the night before to start - there is
no penalty for starting early. Notebook pages are due at
the END of each lab session. The final notebook pages and poster
must be turned in by 5 pm on the due date. Please read the policy
concerning late assignments.
Contributions of individual assignments are as follows:
- 100 points = Scientific Poster (each member of the team
receives the SAME grade)
- 30 points = Lab Notebook (graded ONCE, after the lab ends)
- 40 points = Laboratory Performance
(5 points per day)
- 30 points = Poster Knowledge and Expertise (you will
be asked a question(s) about your poster individually)
- Take advantage of opportunities to earn points; you do not
have as many assignments or as many points as you did in BIOS
311.
- The scientific poster is 50% of your final grade.
Remember, this poster is a TEAM project, and each member will receive the SAME grade.
Therefore, EACH of you is responsible for the ENTIRE poster, not just the part(s) you worked on.
Furthermore, you are expected to understand
the rationale behind all of the experimental procedures
and the meaning of the results.
We will use the Poster Evaluation Form to grade your poster.
Poster CONTENT accounts for 80% of your grade; poster APPEARANCE accounts for 20%.
- The lab notebook is 15% of your final grade.
You learned how to keep a research quality notebook in BIOS 211
and were expected to maintain a research quality notebook in
BIOS 313 and 311. The SAME is expected of you in
BIOS 413; it is YOUR responsibility to review our guidelines
for keeping a proper notebook.
We will use the BIOS 413
Notebook Checklist to
grade your lab notebook.
We intend to use the overall quality of the laboratory notebook and your specific notebook entries to evaluate your individual effort.
A single team member must not, for example, act as a recordkeeper by writing in the notebook while the others carry out the work.
- GRADING SCHEME:
- 95% & up = A+
- 90 - 94% = A
- 87 - 89% = A-
- 84 - 86% = B+
- 81 - 83% = B
- 78 - 80% = B-
- 75 - 77% = C+
- 72 - 74% = C
- 69 - 71% = C-
- 60 - 68% = D
- below 60% = F
- You will be graded against this absolute scale, NOT against your classmates.
- Grades posted on Esther are FINAL and cannot be changed.
- COMMENTS ABOUT LABORATORY PERFORMANCE:
- You will be assessed each week in the following areas:
Preparation and familiarity with procedures
Proper lab notebook (i.e., recording notes DURING experiments, NOT writing on scrap paper and copying at the end of lab or exchanging notebooks with your team at the end of lab)
Teamwork (collaboration, division of labor, troubleshooting, and time management)
Ability to function independently (troubleshooting and time management)
Experimental technique
- You are expected to function both as a member of a TEAM and as an INDEPENDENT investigator.
Follow the experimental procedures, record the results, and analyze/interpret your findings.
Accomplishment of the laboratory goals and preparation and
presentation of the poster require contributions from EACH team
member.
If a team member does not carry his/her weight, focuses only
on his/her own work, or is not competent in the lab please bring
this matter to our attention; be honest and objective (your
comments will remain confidential). A conscientious evaluation
of your peers will be appreciated and will be considered when
determining the laboratory performance grade. On the last
day of lab, you will receive Peer Evaluation Forms so
you can evaluate your team members; please complete these evaluations
and bring them to the poster Q/A session.
- You do not necessarily EARN points for "experimental technique" by
obtaining the expected results (i.e., performing the experiment
properly); likewise, you do not necessarily LOSE points if
you don't obtain the expected results. You are evaluated on
HOW you get the results, not the results themselves.
Sometimes experiments fail, or the results don't make sense, or your findings are at odds with published studies. Because of time constraints and limited materials you may not be able to repeat the procedure in a lab course; however, you should recognize that in a research setting you would perform the experiment again.
- EXAMPLES of how to LOSE Points (this list is NOT all-inclusive):
not preparing for lab (i.e., reading the procedures as you go)
failing to follow explicit instructions in the procedures
failing to come in to complete a procedure (e.g., picking colonies)
showing up late
writing on scratch paper instead of in your notebook
staying after you've finished the procedures to write in your notebook
not using your time efficiently
not working with your team
using the WRONG reagents
not following lab safety rules (e.g., wearing sandals or eating/drinking
in the lab)
talking on your cell phone or the lab phone during lab
****Come to lab prepared and learn to work effectively as a TEAM. Focus on learning and you should do fine.****
Copyright, Acknowledgements,
and Intended Use
Created by B. Beason (bbeason@rice.edu), Rice University, 16 June 2006
Updated 22 April 2009