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SPECIAL NOTE: Record enough procedure details in your notebook during each day of lab so that you can repeat these procedures using your notebook as the ONLY resource (i.e., you should not use printed web pages or handouts from previous labs).

Page construction (5 points)
page numbers (consecutive/no pages missing)
date (all/correct format)
"continued on/from page #"

Maintenance (5 points)
absence of blank pages
appropriate corrections
initial/date/time at end of each entry
table of contents

Objectives / Daily Summary (10 points)
Day 1: model system/overall goals of study
Days 1-8: experimental strategies

Notes During Experiment (10 points)
procedures = WHO/WHAT/HOW??
reagents/supplies
instrument used/settings
samples: labels, storage location/conditions
problems encountered

Raw Data / Results (10 points)
primer design and sequence
qPCR: selection of temperature; specificity; fold changes
RNA quantitation
agarose gels

Discussion / Conclusions (10 points)
primer design
validation of primers for qPCR
RNA isolation
qPCR analysis
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Total score:

Copyright, Acknowledgements, and Intended Use
Created by B. Beason (bbeason@rice.edu), Rice University, 9 July 1999
Updated 14 April 2015