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SPECIAL NOTE: Record enough procedure details in your notebook during each day of lab so that you can repeat these procedures using your notebook as the ONLY resource (i.e., you should not use printed web pages or handouts from previous labs).

Page construction (5 points)
page numbers (consecutive/no pages missing)
date (all/correct format)
"continued on/from page #"

Maintenance (5 points)
absence of blank pages
appropriate corrections
initial/date/time at end of each entry
table of contents

Objectives / Daily Summary (10 points)
model system/overall goals of study
experimental strategies

Notes During Experiment (10 points)
procedures = WHO/WHAT/HOW??
reagents/supplies
instrument used/settings
samples: labels, storage location/conditions
problems encountered

Raw Data and Results (10 points)
agarose gels (PCR, restriction digests)
transformation results/EOT
GUS staining
CML gene sequence analyses (PCR product, primers, T-DNA insertions)

Conclusions (10 points)
creation of GUS reporter gene construct
GUS staining of transgenic plants
primary PCR screen (genotypes of plants)
CML gene sequence analyses
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Total score:

Copyright, Acknowledgements, and Intended Use
Created by B. Beason (bbeason@rice.edu), Rice University, 9 July 1999
Updated 23 April 2009