Discussions, which are required,
are on MONDAYS
from 3:00-4:00 p.m. in Duncan College (DCC) 113 unless
otherwise indicated; labs are scheduled from 1-6
p.m. on Tuesday (section 001) or Wednesday (section 002) in
the Biology basement BCB teaching labs. Please
bring at least 1-2 computers per team to each discussion and
lab.
Pre-lab exercises are submitted in OWL-Space using the "Tests & Quizzes" tool: although there is no time limit for completing the exercises, you are allowed a single submission. Please check OWL-Space for the Release/Due Dates; you must submit your work during this time frame to get full credit for the pre-lab. You must complete the exercises ALONE (i.e., do not work on the pre-lab with anyone else): you may use your class notes/web site/Scopes; have a calculator and pen/pencil with scratch paper on hand as you answer the questions.
You must come to lab prepared--this requires you to READ the experimental protocols on the course web site BEFORE coming to lab, not just print a copy of them and bring it with you. Bring only the information you need to perform the experiments. The procedures for each day are available here and you will
SPECIAL NOTE: Record enough procedure details in your notebook during each day of lab so that you can repeat these procedures using your notebook as the MAIN resource. An exception to this note are the instructions for using the spectrophotometers; there are 3 models, and since you may not always use the same type, you can have those instructions handy in your "BIOC 311 Binder" (for loose data).In this laboratory course, we use several pieces of equipment and many different reagents from Bio-Rad. Check out their web page for useful information on life science research products. On certain lab days you may want to read specific Bio-Rad manuals.
Preparation |
|
Experimental Procedures |
BIOC 311 Honor Code Policy |
|
Buffer preparation |
Project Overview & Team Assignments |
|
ADA stability study |
Enzyme stability and buffer selection |
| |
ADA assay |
|
|
Preparation |
|
Experimental Procedures |
Protein extraction |
|
Pouring of SEC column |
Salt precipitation |
|
Cell lysis & tissue homogenization |
Bradford assay |
|
Bradford assay |
Pre-lab: ADA Elevator Talk |
|
Ammonium sulfate precipitation |
|
Assays of stability samples |
Preparation |
|
Experimental Procedures |
Size exclusion chromatography theory |
|
Size exclusion chromatography |
Detection of ADA activity |
|
Qualitative ADA activity assays |
Dialysis |
|
Selection/preparation of anion exchange buffer |
Buffers for ion exchange |
|
Dialysis |
ADA stability study results |
|
|
Preparation |
|
Experimental Procedures |
Plagiarism and Research Paper overview |
|
Anion exchange chromatography |
Writing the Materials and Methods |
|
Qualitative and quantitative ADA activity assays |
Anion exchange chromatography set-up |
|
Desalting Q fraction |
Protein determination |
| |
Protein purification tables |
|
Preparation |
|
Experimental Procedures |
Writing the Introduction |
|
Kinetic studies |
Spectrophotometry |
|
|
Kinetics review |
|
|
Preparation |
|
Experimental Procedures |
Writing the Results |
|
SDS-PAGE |
Electrophoresis applications |
|
Silver stain of SDS-PAGE gel |
|
Electrophoretic transfer |
Preparation |
|
Experimental Procedures |
Writing the Discussion and Abstract |
|
Western blot development |
Isoelectric focusing |
|
Isoelectric focusing |
Coupled enzyme assays |
|
Activity stain of IEF gel |
Western blot development |
|
Copyright, Acknowledgements,
and Intended Use
Created by B. Beason (bbeason@rice.edu), Rice University, 10 June 1999
Updated 3 January 2014