Home

SPECIAL NOTE: Record enough procedure details in your notebook during each day of lab so that you can repeat these procedures using your notebook as the PRIMARY resource (i.e., you should not use printed web pages or handouts from previous labs). Store additional data (e.g., gel pictures, Excel spreadsheets, and graphs) in your 313 folder (do not tape them into your lab notebook); refer to them in your lab notebook and indicate the names of electronic files.

Page construction (5 pts)
page numbers (consecutive/no pages missing)
date (all/correct format)
"continued from/on page #"

Maintenance (5 pts)
absence of blank pages
appropriate corrections
initial/date/time at end of each entry
table of contents (appropriate detail)

Objectives / Summary (5 pts)

Notes during experiment (5 pts)
procedures = WHO/WHAT/HOW??
reagents/supplies
instrument used/settings
samples: labels, storage location/conditions
problems encountered / mistakes

Raw data & Analysis/Conclusions (10 pts)
Construction of a simple circuit: were you successful?

• agarose gel analysis of restriction enzyme digests of BioBricks
• DNA ligation of BioBricks and bacterial transformation
• colony PCR screen: agarose gel analysis of PCR (size of products)

DNA design

• primer design and PCR of plasmid DNA to mutate RBS
• agarose gel analysis and DpnI digestion
• transformation

Functional analysis of simple circuit

• use of plate reader
• brief description of fluorescence analysis results

Total score: