Books and Book Chapters:

1. T.S.Tkaczyk, book chapter: "Endomicroscopy" in “Biomedical Optical Imaging Techniques: Design and Applications”, Springer, 2013, XIV, 380 p. 155 illus.,

2. T.S. Tkaczyk, Field Guide to Microscopy SPIE Press, 2010.

3. T.Milster and T.S.Tkaczyk, book chapter: “Miniature and Micro-Optics” in Handbook of Optics, vol. 1, McGraw Hill, October 2009.

Journal Papers:


1. Cynthia Wong, Michal E. Pawlowski, Alessandra Forcucci, Catherine E. Majors, Rebecca Richards-Kortum, Tomasz S. Tkaczyk. Development of a universal, tunable, miniature fluorescence microscope for use at the point of care. Biomed. Opt. Express 9(3):1041-1056 (March 1, 2018).


1. Amicia D. Elliott, Noah Bedard, Alessandro Ustione, Michelle A. Baird, Michael W. Davidson, Tomasz Tkaczyk, David W. Piston. Hyperspectral imaging for imultaneous measurements of two FRET biosensors in pancreatic beta-cells. PLoS ONE. 12(12):e0188789 (December 6, 2017).

1. John Gawedzinski, Michal E. Pawlowski, Tomasz S. Tkaczyk. Quantitative evaluation of performance of three-dimensional printed lenses. Opt. Eng. 56(8):084110 (Aug 30, 2017).

2. Ye Wang, Michal E. Pawlowski, Tomasz S. Tkaczyk. High spatial sampling light-guide snapshot spectrometer. Opt. Eng. 56(8):1-12 (Mar 23, 2017).

3. Jason G. Dwight, Tomasz S. Tkaczyk. Lenslet array tunable snapshot imaging spectrometer (LATIS) for hyperspectral fluorescence microscopy. Biomed. Opt. Express 8(3):1950-1964 (Mar 1, 2017).

4. Adam Shadfan, Hawraa Darwiche, Jesus Blanco, Ann Gillenwater, Rebecca Richards-Kortum, Tomasz S. Tkaczyk. Development of a multimodal foveated endomicroscope for the detection of oral cancer. Biomed. Opt. Express 8(3):1525-1535 (Mar 1, 2017).

5. Kaushik Subramanian, Ilan Gabay, Onur Ferhanoglu, Adam Shadfan, Michal Pawlowski, Ye Wang, Tomasz Tkaczyk, Adela Ben-Yakar. A Kagome fiber-based, high energy delivery laser scalpel system for ultrafast laser microsurgery. Energy-based Treatment of Tissue and Assessment IX. Proc. SPIE 10066:1-11 (Feb 22, 2017).


1. Kaushik Subramanian, Ilan Gabay, Onur Ferhanoglu, Adam Shadfan, Michal Pawlowski, Ye Wang, Tomasz Tkaczyk, Adela Ben-Yakar. Kagome fiber based ultrafast laser microsurgery probe delivering micro-Joule pulse energies. Biomed. Opt. Express 7(11):4639-4653 (Nov 1, 2016).

2. Cynthia Wong, Ngan P. Ha, Michal E. Pawlowski, Edward A. Graviss, Tomasz S. Tkaczyk. Differentiating between live and dead Mycobacterium smegmatis using autofluorescence. Tuberculosis. Articles in Press. (Fall 2016).

3. Jason G. Dwight, Christina Y. Weng, Robert E. Coffee, Michal E. Pawlowski, Tomasz S. Tkaczyk. Hyperspectral Image Mapping Spectrometry for Retinal Oximetry Measurements in Four Diseased Eyes. International Ophthalmology Clinics. 56(4):25-28. (Fall 2016).

4. Zeno Lavagnino, Jason Dwight, Alessandro Ustione, Thuc-Uyen Nguyen, Tomasz S. Tokaczyk, David W. Piston. Snapshot Hyperspectral Light-Sheet Imaging of Signal Transduction in Live Pancreatic Islets. Biophysical Journal. 111(2):409-417. (July 26, 2016).

5.Brian McCall, Mark Pierce, Edward A. Graviss, Rebecca R. Richards-Kortum, Tomasz S. Tkaczyk. Miniature objective lens for array digital pathology: design improvement based on clinical evaluation. Medical Imaging. Proc. SPIE 9791:1-6 (Mar 23, 2016).

6.Jessica Dobbs, Matthew Kyrish, Savitri Krishnamurthy, Benjamin Grant, Henry Kuerer, Wei Yang, Tomasz Tkaczyk, Rebecca Richards-Kortum. High resolution microendoscopy with structured illumination and Lugol's iodine staining for evaluation of breast cancer architecture. Optical Biopsy XIV: Towards-Real-Time Spectroscopic Imaging and Diagnosis. Proc. SPIE 9703:1-11 (Mar 7, 2016).

7. Adam Shadfan, Michal Pawlowski, Ye Wang, Kaushik Subramanian, Ilan Gabay, Adela Ben-Yakar, Tomasz Tkaczyk. Design and fabrication of a miniature objective consisting of high refractive index zinc sulfide lenses for laser surgery. Optical Engineering. 55(2). (Feb 23, 2016).

8. Adam H. Shadfan, Michal E. Pawlowski, Tomasz S. Tkaczyk. Development of tunable miniature piezoelectric-based scanners validated by the combination of two scanners in a direct image relay. Opt. Eng. 55(1) 013104 (Jan 18, 2016).


1. Soren D. Konecky ; Robert H. Wilson ; Nathan Hagen ; Amaan Mazhar ; Tomasz S. Tkaczyk, Ron D. Frostig, and Bruce Tromberg, "Hyperspectral optical tomography of intrinsic signals in the rat cortex", Neurophoton. 2(4), 045003 (Nov 12, 2015).

2. Alessandra Forcucci, Michal Pawlowski, Zachary Crannell, Catherine Majors, Ina Pavlova, Rebecca Richards-Kortum, and Tomasz S. Tkaczyk, 3D printed miniature fluorescence microscope for point-of-care readout of bead-based bioassays, J. Biomed. Opt. 20 (10), 105010 (October 21, 2015);

3. Shalini Jain, Xiao Wang, Chia-Chi Chang, Catherine Ibarra-Drendall, Hai Wang, Qingling Zhang, Samuel W. Brady, Ping Li, Hong Zhao, Jessica Dobbs, Matt Kyrish, Tomasz S. Tkaczyk, Adrian Ambrose, Christopher Sistrunk, Banu Arun, Rebecca Richards-Kortum, Wei Jia, Victoria L. Seewaldt, and Dihua Yu, “Src Inhibition Prevents Estrogen Receptor-Negative Breast Cancer by Blocking c-Myc Translation and Glucose Metabolism”, Cancer Res, September 17, 2015.

4. Alessandra Forcucci, Michal E. Pawlowski, Catherine Majors, Rebecca Richards-Kortum, and Tomasz S. Tkaczyk, "All-plastic, miniature, digital fluorescence microscope for three part white blood cell differential measurements at the point of care," Biomed. Opt. Express 6, 4433-4446 (2015)

5. Adam Shadfan, Anne Hellebust, Rebecca Richards-Kortum, and Tomasz Tkaczyk, Confocal foveated endomicroscope for the detection of esophageal carcinoma, Vol. 6, Issue 7, pp. 2311-2324 (2015)

6. Michal E. Pawlowski, Sebina Shrestha, Jesung Park, Brian E. Applegate, John S. Oghalai, and Tomasz S. Tkaczyk, Miniature, minimally invasive, tunable endoscope for investigation of the middle ear, Vol. 6, Issue 6, pp. 2246-2257 (2015)

7. PA. Keahey, T.Tkaczyk, KM. Schmeler, and RR. Richards-Kortum, "Optimizing modulation frequency for structured illumination in a fiber-optic microendoscope to image nuclear morphometry in columnar epithelium," Biomed. Opt. Express 6, 870-880 (2015)


1. Krzysztof Patorski, Maciej Trusiak, and Tomasz Tkaczyk, "Optically-sectioned two-shot structured illumination microscopy with Hilbert-Huang processing," Opt. Express 22, 9517-9527 (2014)

2. Mark C. Pierce, Shannon E. Weigum, Jacob M. Jaslove, Rebecca Richards-Kortum, Tomasz S. Tkaczyk, Optical Systems for Point-of-care Diagnostic Instrumentation: Analysis of Imaging Performance and Cost, Annals of Biomedical Engineering, January 2014, Volume 42, Issue 1, pp 231-240


1. Matthew Kyrish ; Jessica Dobbs ; Shalini Jain ; Xiao Wang ; Dihua Yu ; Rebecca Richards-Kortum ; Tomasz S. Tkaczyk, "Needle-based fluorescence endomicroscopy via structured illumination with a plastic, achromatic objective", J. Biomed. Opt. 18(9), 096003 (Sep 03, 2013). doi:10.1117/1.JBO.18.9.096003

2.Brian McCall, Randall J. Olsen, Nicole J. Nelles, Dawn L. Williams, Kevin Jackson, Rebecca Richards-Kortum, Edward A. Graviss, and Tomasz S. Tkaczyk, "Evaluation of a Miniature Microscope Objective Designed for Fluorescence Array Microscopy Detection of Mycobacterium tuberculosis". Archives of Pathology & Laboratory Medicine, Published Online: August 15, 2013.

3. Thuc-Uyen Nguyen, Mark C Pierce, Laura Higgins, and Tomasz S Tkaczyk, "Snapshot 3D optical coherence tomography system using image mapping spectrometry," Opt. Express 21, 13758-13772 (2013)

4. Noah Bedard, Richard A. Schwarz, Aaron Hu, Vijayashree Bhattar, Jana Howe, Michelle D. Williams, Ann M. Gillenwater, Rebecca Richards-Kortum, and Tomasz S. Tkaczyk, "Multimodal snapshot spectral imaging for oral cancer diagnostics: a pilot study," Biomed. Opt. Express 4, 938-949 (2013)

5. Meaghan Bond, Carlos Elguea, Jasper S. Yan, Michal Pawlowski, Jessica Williams, Amer Wahed, Maria Oden, Tomasz S. Tkaczyk and Rebecca Richards-Kortum, “Chromatography paper as a low-cost medium for accurate spectrophotometric assessment of blood hemoglobin concentration”, Lab Chip, 2013, 13, 2381–2388,

6. Brian McCall and Tomasz S. Tkaczyk, "Rapid fabrication of miniature lens arrays by four-axis single point diamond machining," Opt. Express 21, 3557-3572 (2013)

7. Matthew Kyrish and Tomasz S. Tkaczyk, "Achromatized endomicroscope objective for optical biopsy", Biomedical Optics Express, Vol. 4, Issue 2, pp. 287-297 (2013)


1. Noah Bedard, Timothy Quang, Kathleen Schmeler, Rebecca Richards-Kortum, and Tomasz S. Tkaczyk, "Real-time video mosaicing with a high-resolution microendoscope," Biomed. Opt. Express 3, 2428-2435 (2012),

2. Noah Bedard and Tomasz S. Tkaczyk, "Snapshot spectrally encoded fluorescence imaging through a fiber bundle", Journal of Biomedical Optics Letters, August 2012 • Vol. 17(8), p.080508-1

3. Amicia D Elliott, Liang Gao, Alessandro Ustione, Noah Bedard, Robert Kester, David W Piston, and Tomasz S. Tkaczyk, Real-time Hyperspectral Fluorescence Imaging of Pancreatic beta-Cell Dynamics with the Image Mapping Spectrometer (IMS), Journal of Cell Science, JOCES/2012/108258

4. Noah Bedard, Nathan Hagen, Liang Gao, Tomasz S. Tkaczyk, "Image mapping spectrometry: calibration and characterization", Optical Engineering 51(11), (November 2012),

5. Nathan Hagen, Robert T. Kester, Liang Gao, Tomasz S. Tkaczyk, " The snapshot advantage: a review of the improvement in light collection for parallel high-D measurement systems" Optical Engineering, Optical Engineering 51(11), 111702 (November 2012)

6. Liang Gao and Tomasz S. Tkaczyk, "Correction of vignetting and distortion errors induced by two-axis light beam steering", Optical Engineering 51(4), 043203 (April 2012)

7. Nathan Hagen and Tomasz S. Tkaczyk, Foveated endoscopic lens, J. Biomed. Opt. 17, 021104 (Mar 12, 2012);

8. Liang Gao, Nathan Hagen and Tomasz S . Tkaczyk, "Quantitative comparison between full-spectrum and filter-based imaging in hyperspectral fluorescence microscopy", JOURNAL OF MICROSCOPY Volume: 246 Issue: 2 Pages: 113-123, MAY 2012

9. Nathan Hagen, Liang Gao, and Tomasz S. Tkaczyk, "Quantitative sectioning and noise analysis for structured illumination microscopy" OPTICS EXPRESS Volume: 20 Issue: 1 Pages: 403-413, 2012

10. Liang Gao, R. Theodore Smith, and Tomasz S. Tkaczyk, "Snapshot hyperspectral retinal camera with the Image Mapping Spectrometer (IMS)," Biomed. Opt. Express 3, 48-54 (2012)


1. Brian McCall, Mark Pierce, Edward A. Graviss, Rebecca Richards-Kortum, Tomasz S. Tkaczyk, Toward a Low-cost Compact Array Microscopy Platform for Detection of Tuberculosis, Tuberculosis 91 (2011) S54-S60.

2. Liang Gao, Noah Bedard, Nathan Hagen, Robert T. Kester, and Tomasz S. Tkaczyk, "Depth-resolved image mapping spectrometer (IMS) with structured illumination," Opt. Express 19, 17439-17452 (2011)

3. N.Hagen and T.Tkaczyk, Compound prism design principles, I, APPLIED OPTICS / Vol. 50, No. 25 / 1 September 2011.

4. N.Hagen and T.Tkaczyk, Compound prism design principles, II: triplet and Janssen prisms, APPLIED OPTICS / Vol. 50, No. 25 / 1 September 2011,

5. N.Hagen and T.Tkaczyk, Compound prism design principles, III: linear-in-wavenumber and OCT prisms, APPLIED OPTICS / Vol. 50, No. 25 / 1 September 2011,

6. Robert T. Kester, Noah Bedard, Liang Gao, Tomasz S. Tkaczyk, Real-Time Snapshot Hyperspectral Imaging Endoscope, Journal of Biomedical Optics, 16(05), 056005, May 2011.

7. Matthew Kyrish, Urs Utzinger, Michael R. Descour, Brenda K. Baggett, and Tomasz S. Tkaczyk, "Ultra-slim plastic endomicroscope objective for non-linear microscopy", Opt. Express 19, 7603-7615 (2011).


1. Liang Gao, Robert T. Kester, Nathan Hagen, and Tomasz S. Tkaczyk , "Snapshot image-mapping spectrometer for hyperspectral fluorescence microscopy", OPN 21:12, 50 (2010).

2. Liang Gao, Robert T. Kester, Nathan Hagen, Tomasz S. Tkaczyk, "Snapshot Image Mapping Spectrometer (IMS) with high sampling density for hyperspectral microscopy", Opt. Express 18, 14330-14344 (2010).

3. Robert T. Kester, Liang Gao, and Tomasz S. Tkaczyk, "Development of image mappers for hyperspectral biomedical imaging applications", Appl. Opt. 49, 1886-1899 (2010).

4. Sara M. Landau, Chen Liang, Robert T. Kester, Tomasz S. Tkaczyk, and Michael R. Descour, "Design and evaluation of an ultra-slim objective for in-vivo deep optical biopsy", Opt. Express 18, 4758-4775 (2010).

5. Brian McCall and Tomasz S. Tkaczyk, “Fabrication of plastic microlens array for array microscope by three-dimensional diamond micromilling,” Opt. Eng. 49, 103401 (2010).

6. Liang Gao, Robert T. Kester, Nathan Hagen, and Tomasz S. Tkaczyk, "Snapshot image-mapping spectrometer for hyperspectral fluorescence microscopy," Opt. Phot. News 50(12), 50 (2010).


1. Liang Gao, Robert T. Kester, and Tomasz S. Tkaczyk, "Compact Image Slicing Spectrometer (ISS) for hyperspectral fluorescence microscopy", Opt. Express 17, 12293-12308 (2009).

2. Robert T. Kester, Todd Christenson, Rebecca Richards Kortum, and Tomasz S. Tkaczyk, "Low cost, high performance, self-aligning miniature optical systems", Appl. Opt. 48, 3375-3384 (2009).


1. Jeremy D. Rogers, Sara Landau, Tomasz S. Tkaczyk, Michael R. Descour, Mohammed S. Rahman, Rebecca Richards-Kortum, Ari H. O. Kärkäinen, Todd Christenson, "Imaging performance of a miniature integrated microendoscope" J. Biomed. Opt. 13, 054020 (2008).


1. Robert T. Kester, Tomasz Tkaczyk, Michael R. Descour, Todd Christenson, and Rebecca Richards-Kortum, "High numerical aperture microendoscope objective for a fiber confocal reflectance microscope," Opt. Express 15, 2409-2420 (2007).


1. H. Luo, T. Tkaczyk, E. L. Dereniak, K. Oka, and R. Sampson, "High birefringence of the yttrium vanadate crystal in the middle wavelength infrared," Opt. Lett. 31, 616-618 (2006).

2. J. Rogers, T. Tkaczyk, M. Descour, A. Kärkkäinen, and R. Richards-Kortum, "Removal of ghost images by using tilted element optical systems with polynomial surfaces for aberration compensation," Opt. Lett. 31, 504-506 (2006).

3. Haitao Luo, Kazuhiko Oka, Nathan Hagen, Tomasz Tkaczyk, and Eustace L. Dereniak, "Modeling and optimization for a prismatic snapshot imaging polarimeter," Appl. Opt. 45, 8400-8409 (2006).


1. M. Rahman, M. Abd-El-Barr, V. Mack, T. Tkaczyk, K. Sokolov, R. Richards-Kortum, M. Descour, “Optical imaging of cervical pre-cancers with structured illumination: An integrated approach”, Gynecologic Oncology 99 (3): S112-S115 Suppl. 1, (2005)


1. Tomasz S. Tkaczyk, Mohammed Rahman, Vivian Mack, Konstantin Sokolov, Jeremy D. Rogers, Rebecca Richards-Kortum, Michael R. Descour, “High resolution, molecular-specific, reflectance imaging in optically dense tissue phantoms with structured-illumination”, Opt. Express 12, 3745 (2004).

2. Siddharth S. Rege, Tomasz S. Tkaczyk, Michael R. Descour, “Application of the Alvarez-Humphrey concept to the design of a miniaturized scanning microscope”, Opt. Express 12, 2574-2588 (2004).

3. Jeremy D. Rogers, Ari H. O. Kärkkäinen, Tomasz Tkaczyk, Juha T. Rantala, and Michael Descour, "Realization of refractive microoptics through grayscale lithographic patterning of photosensitive hybrid glass," Opt. Express 12, 1294-1303 (2004).


1. T.Tkaczyk, R.Jóźwicki, “Full field heterodyne interferometer for shape measurement - experimental characteristics of the system”, Optical Engineering, vol. 42, no. 8, p. 2391-2399, 2003,

2. K. W. Gossage, T. Tkaczyk, J. J. Rodriguez, and Jennifer K. Barton, “Texture analysis of optical coherence tomography images: feasibility for tissue classification”, J. Biomed. Opt. 8, 570-575 (2003).

2002 and earlier

1.T.Tkaczyk; R.Jozwicki "Influence of optical imaging on phase measurements in fringe projection coherent systems", Opt. Eng. 41, 811-821 (2002).

2. L.Wronkowski; T.Tkaczyk; A.Olszak Opto-electronic displacement gauge based on speckle interferometer, Optics and Lasers in Engineering. vol.32, no.1; p.65-77, 1999.

3. B.Mroziewicz; B.Janowska-Dmoch; T.Tkaczyk; M.Gorska, A novel method of recording high frequency circular gratings, Optics and Laser Technology. vol.31, no.4, p.315-24, 1999.

4. R.Jozwicki; T.Tkaczyk, “Problems related to numerical determination of the image given by aberrated optical system under the coherent illumination. Educational aspects”, Optica Applicata. vol.29, no.1-2, p.251-6, 1999.

5. T.Tkaczyk; B.Mroziewicz, “Semiconductor corrugated surface structures produced using interference method”, Optica Applicata. vol.26, no.3, p.185-99, 1996.


Selected Invited Talks:

1. T.Tkaczyk, Low-Cost Optical Diagnostic Systems for Point of Care Applications – point of care cost effective integrated optics, Frontiers in Optics, OSA Annual Meeting, October 2015

2. T.Tkaczyk, Novel Mechatronic Strategies Toward Development of Miniature Biomedical Devices, Plenary talk at International Mechatronics Conference, Warsaw, September 2015.

3. T.Tkaczyk, Integrated Optics for Biomedical Imaging Applications and Hyperspectral Snapshot Modalities, Applied Industrial Optics (AIO), OSA, June 2015

4. T.Tkaczyk, Multimodal imaging strategies for early cancer diagnostics Molecular-Guided Surgery, Photonics West, San Francisco, February 2015

5. T.Tkaczyk, “Low-Cost Optical Diagnostic Systems for Global Health", Gordon Research Conferences, Lasers in Medicine and Biology, NH, July 2014.

6. T.Tkaczyk, “Miniature Sensing Devices for Biomedical Applications”, Tutorial during Pre-Conference Workshop, Biosensors 2014, Melbourne, Australia

7. T.TKaczyk, "Cost effective, high performance optics for POC applications", Frontiers in Optics Conference, Annual Meeting of Optical Society of America, , Rochester, NY, October, 2012,

8. T.Tkaczyk, "Multi-dimensional snapshot imaging using field distribution techniques", Optical Society of America Conference on Computational Optical Sensing and Imaging (COSI), Monterey, CA, June 2012

9. T.Tkaczyk, "Cost estimation and cost effective technologies for building POC optical devices", BioMedOpTex conference, May 2012, Collage Station, TX

10. T.Tkaczyk, “Image mapping spectrometry – a novel hyperspectral platform for rapid snapshot imaging”, Defense and Security, SPIE International Conference, Orlando, April 2011.

11. T.Tkaczyk, “Roadmap for Multi-Modal Miniature Microscopy on its path from bench to the bedside”, SPIE organized Inter-Institute Workshop on Optical Diagnostic and Biophotonic Methods from Bench to Bedside, Bethesda, October 2009.

12. T.Tkaczyk, Miniature Microscopy for Bio-Medical Applications, SPIE Photonics West 2009, San Jose, CA T.Tkaczyk, “Confocal Microendoscopy”, at Advances in Optics for Biotechnology, Medicine and Surgery, ECI International Conference, Naples, FL, USA, June 2007.

13. T.Tkaczyk, “Modern technologies to build inexpensive, high performance micro-endoscopes for cellular imaging”, at Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications VIII, SPIE BIOS Conference, San Jose, CA, USA, January 2007.

14. T. Tkaczyk, “Miniaturized, disposable microscopy probes for early cancer detection”, Optical Imaging 2006, Fifth Inter-Institute Workshop on Optical Diagnostic Imaging from Bench to Bedside, National Institutes of Health, Bethesda, Maryland USA, September 2006

15. T.Tkaczyk, “Current trends in optical detection of early cancer stages”, 5th European Syphosium on Biomedical Engineering, Patras, Greece, July 2006.

16. T.Tkaczyk, “Multi-modal miniaturized microscope: successful merger of optical, MEMS, and electronic technologies”, Optomechatronic Technologies (ISOT 2005), Sapporo (Japan), December 2005.