A. Basic laboratory skills
ability to measure and report uncertain quantities
with appropriate precision
Uncertain quantities are any measured or derived numbers
that must be rounded to an appropriate number of significant
figures. Every physics problem you have ever worked should
have required appropriate rounding of answers. All teaching
laboratory courses that involve quantitative work will
require the same. Despite all of this emphasis, many
of you persistently ignore uncertainty when reporting
data and calculations.
Example. A 1% (w/v) solution is defined as 1
gram solute in 100 milliters solvent, final volume. It
is easy to see that 3 gms solute in 100 ml final volume
is a 3% solution. Bu suppose you have 10 gms solute in
700 ml final volume. If you would report having a 1.42857143%
solution, then you need to work on this concept.
ability to convert raw data to a physically meaningful
form
Conversion of raw data to a physically meaningful form
refers to selection of appropriate units and to convert
from measured quantities with arbitrary units to quantities
with units that have universal significance.
For example, you should be able to read raw sensor
data from a chart with a time axis, and convert a slope
to a rate in meaningful units versus time. Another scientist
will know what you mean if you report a rate in millimoles
molecular oxygen generated per minute, but will get no
useful information out of "number of squares per inch
of chart record."
ability to apply appropriate methods of analysis
to raw data
Appropriate methods refers to your choice of an analytical
method, such as whether or not to plot data, appropriate
plot type, scale, and units, and choice of statistical
analysis if appropriate. You should always be prepared
to represent experimental error when appropriate.
ability to carry out common laboratory procedures
correctly
Examples include pipetting or weighing in a chemistry
or biosciences laboratory, cell transfer in a bioengineering
lab, and wiring a circuit or using an oscilloscope in
physics.
ability to adhere to instructions on laboratory
safety and to recognize hazardous situations and act
appropriately
To the extent that our laboratory coursework involves
hazards, we ask you to adhere to specific safety rules,
including both proper attire and behavior. Industrial
and government laboratories, as well as most medical
institutions, have very high standards for saftety practices.
We also ask you to exercise common sense when faced with
potentially hazardous situations.
ability to perform logical troubleshooting of laboratory
procedures
Examples include checking intermediate steps in any
protocol, and testing a procedure with known inputs,
such as using a wave generator to test a circuit or using
a known protein mixture to test an assay solution. As
you progress through our laboratory courses you should
eventually be able to solve your own problems without
having to get an instructor's help.
B. Communication and record
keeping
ability to maintain an up-to-date laboratory notebook
(including proper documentation of outside resources)
which is of sufficient detail that others could repeat
any of your experiments if necessary
You should be in the habit of documenting all laboratory
procedures completely and at the time you conduct them,
in chronological order. Any information needed to write
up an experiment should be in your notebook, and a competent
scientist who is unfamiliar with the work should be able
to read your notebook, make sense of what you did, and
be able to repeat the experiments. Document sources,
such as original publications you used as a source of
methods, in your notebook.
ability to talk about your results in a clear and
concise manner
You should be able to summarize and discuss a protocol.
You should also be able to orally answer questions about
a procedure or piece of equipment in the laboratory.
If you have good speaking skills you will have an advantage
in almost any undertaking.
To make a good oral presentation, you need to clearly
present all necessary information to an uninformed audience
in an effective (straightforward, dynamic) manner. One
should be able to talk about a familiar subject at the
level that is appropriate for the audience. Experience
in presenting posters is also valuable particularly if
you are considering post-graduate research. To the extent
that poster presentation forms a basis for discussion
of ongoing work, this is a type of oral communication.
ability to write effectively in appropriate style
and depth
We expect you to have fundamental writing skills upon
entry into the program, including the ability to properly
design paragraphs and to organize ideas in paragraph
form. If you are not confident in your writing skills
you may want to consider taking a course in English composition
or technical writing.
In addition to fundamental writing skills, technical
writing requires that you be able to
- introduce a paper, providing a full and complete
rationale for a study, including clear statements of
hypothesis(es), objectives, and significance of the
work.
- convert details, such as specific procedures, to
a general process; for example, write up a concise
materials and methods section that includes only the
minimal information needed to ensure reproducibility
of the work
- prepare tables, figures, and graphs that succinctly
summarize critical data; appropriate statistical analysis
may need to be included.
- organize discussion of data effectively, including
explanations of the relationship of each figure, table,
and/or set of data to the objectives of a study.
- address one or more questions in a well-organized
discussion, incorporating results into the discussion,
clearly distinguishing new information and/or speculation
from established facts and theories
- present concepts in sufficient depth to provide a
full and complete explanation.
- write up a complete and concise summary (abstract)
for a paper, including all major results and conclusions,
supported by quantitative data if applicable.
- exercise economy of words and avoid redundancy. For
example, a figure legend should complement the text
rather than repeat it.
ability to access relevant information from the
library and other information resources
You should be able to use search indexes, find journals,
select and organize appropriate references, cite references
in a paper, and prepare a bibliography or list of literature
cited.
C. Maturity and responsibility
ability to effectively prepare in advance for laboratory
work (extended, of course, to any undertaking that
requires advance preparation)
ability to learn from mistakes
In introductory courses most of the mistakes you make
will be on assignments such as quizzes, calculations,
lab reports, and the like. We expect you to accept criticism
and take suggestions, showing improvement the next time
you encounter a similar situation.
As you progress throughout the program, we expect you
to become self-critical and to take personal responsibility
for learning.
ability to take the initiative and work independently
You won't always have a lab partner to lean on. It is
important that you be able to identify an objective,
determine for yourself how to accomplish it, and carry
out the work yourself.
ability to work effectively as part of a team
While individual initiative is essential there is no
way to get around the fact that most science in today's
world requires collaboration with others. For most of
us, working well with others is crucial for success.
In a teaching lab, you and one or more other students
should work effectively as a team, dividing up responsibilities,
and complete lab work in a timely manner. You should
be able to work effectively with students of different
backgrounds and abilities.
D. Context
ability to understand your data and to report data
effectively
Data are meaningless without interpretation. Understanding
what your data say and, just as importantly, what they
do not say, is crucial. A failure to account for a single
variable in your experimental design could turn your "good" data
set into a bad one. Conversely, there is often gold to
be mined in a "bad" data set if it is onlyu
viewed from a slightly different perspective.
The beginning student typically feels compelled to present
all of his/her data in a written report. Often this stems
from a belief that the instructor needs "proof" that
the student actually did the required work. However,
unless the data set is small and not summarized in some
other form (through graphs or summary statistics), raw
data are rarely reported in the scientific literature.
Likewise, most of your lab instructors don't want to
see your raw data in your lab reports either. Instead,
you will be asked to refine your raw data into a form
that is both easily understandable and pertinent to the
question you are addressing. This may take the form of
a graph or summary statistics. Don't clutter your otherwise
polished report with a mass of raw data!
Please note, though, that the need to convert data is
not a license for bad record keeping. You should always
be able to produce your raw data if asked.
Note: there's also a question of economy that I'm not
addressing here that you might want to (for example,
making sure that figure legends complement text rather
than repeat it).
ability to relate laboratory work to the bigger
picture, to recognize the applicability of scientific
principles to real world situations, and to recognize
when seemingly minor oversights can have serious consequences
A quantitative error in determining a drug dosage can
kill. If you are considering a health profession please
consider the significance of misreporting a quantity
by one or more orders of magnitude. Misspelling of a
chemical name can change the meaning completely. You
can ruin an experiment, ruin a product, and/or cause
someone serious harm by mistakenly changing one letter
of a chemical compound. You may not think that physics
applies to real life, but consider how time and distance
relationships as described by Newtonian physics apply
to driving a motor vehicle.
ability to explain what is meant by the scientific
method
To explain what is meant by the scientific method you
need to understand:
- the scientists' definition of theory
- what are, in a conceptual sense, the qualities of
a good hypothesis
- the role of experimental design in testing a hypothesis
- the definition of random error and how scientists
account for it
Many people think that scientists first take a position
then try to "prove" it experimentally. You need to fully
understand how fallacious that conception is. How can
one make a new discovery if one already thinks he/she
has the answer?
E. Integration and application of
knowledge/experience
ability to integrate and apply information and experience
from math and science courses to current and future
work
This refers to compartmentalized learning, and is one
of the most critical objectives to be met. Facts, methods,
and principles that you have learned in one course often
apply to another course, even in a completely different
discipline. You cannot fully understand the principals
of chemistry without some fundamentals in physics and
mathematics. All three disciplines are essential to anyone
in life sciences. Coursework is arranged in separate
disciplines for convenience, not because there is a real
separation. Please try to retain what you have learned
in each of your courses. You will need it later.
ability to apply critical thinking in the laboratory
Critical thinking in this context refers to strategic
decision-making based upon observations made in the laboratory,
as opposed to simple troubleshooting of experimental
procedures. You need to be able to make observations
in the laboratory, draw conclusions, and act upon those
conclusions.
ability to recognize whether results and conclusions "make
sense"
You can avoid making many mistakes by learning to estimate.
Learn to determine if calculated or measured values are
within a reasonable range. For example, the velocity
of flight of a bird is not reasonable if it is greater
than the speed of light. You should be able to understand
your results and conclusions in the context of other
published work.
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