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When you come to the end of your rope,
tie a knot and hang on.
Franklin D. Roosevelt

Day 4: Validation of primers for qPCR

Pre-Lab Topics

Experimental Procedures

Overview of Procedures

We are going to isolate plasmid DNA from bacterial transformants.
We will perform restriction digests to confirm that the clone contains the GUS vector with the CML 5'-URS insert.

Experimental Procedures

qPCR

Copyright, Acknowledgements, and Intended Use
Created by B. Beason (bbeason@rice.edu), Rice University, 23 July 2003
Updated 20 July 2011