When you come to the end of your rope,
tie a knot and hang on.
Franklin D. Roosevelt

Day 2: Validation of primers for qPCR

Discussion Topics

Experimental Procedures

Overview of Procedures

We're using SsoAdvanced™ SYBR® Green Supermix from for the qPCR reactions.  We are going to validate our primers by performing gradient PCR (vary temperature and generate melt curve).

Copyright, Acknowledgements, and Intended Use
Created by B. Beason (bbeason@rice.edu), Rice University, 23 July 2003
Updated 12 March 2014