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BIOS 313 Notebook Checklist

SPECIAL NOTE: Record enough procedure details in your notebook during each day of lab so that you can repeat these procedures using your notebook as the ONLY resource (i.e., you should not use printed web pages or handouts from previous labs).

Page construction (5 pts)
page numbers (consecutive/no pages missing)
date (all/correct format)
"continued from/on page #"

Maintenance (5 pts)
absence of blank pages
appropriate corrections
initial/date/time at end of each entry
table of contents

Objectives / Daily Summary (10 pts)
overview/goals of study
experimental strategies

Notes during experiment (10 pts)
procedures = WHO/WHAT/HOW??
reagents/supplies
instrument used/settings
samples: labels, storage location/conditions
problems encountered

Raw data & Calculations (10 pts)
DNA quantitation (A260 vs. EtBr gel)
agarose gels (RE digests, PCR)
transformation results/EOT
precision/significant figures

Analysis / Conclusions (10 pts)
Cells and DNA
DNA manipulations
Subcloning BioBricks
PCR amplification
Construction of a simple circuit: were you successful?

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Total score:


Copyright, Acknowledgements, and Intended Use
Created by B. Beason (bbeason@rice.edu), Rice University, 10 January 2008
Updated 11 September 2009