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BIOS 313 Notebook Checklist
Each major item will be scored from 0 to 5.
Page construction
page numbers (all)
date (all)
"continued from/on page #"
Maintenance
absence of blank pages
appropriate corrections
initial/date/time at end of each entry
table of contents
Purpose / Introduction
model system
overview/goals of study
experimental strategies
Notes during experiment
- model system
- experimental treatments
- microarray slides: source, I.D. #
- problems encountered
Preparation and Labeling of cDNA
- Reverse transcription reaction
- Prehybridization/Hybridization
- 3DNA labeling
Scanning
- settings
- analyses
Image Analysis
- aligning blocks
- flagging features
- identifying genes
Raw data
scan results
analysis report
scatter plots
Summary and Conclusions
Hybridization of microarray chip
- Any problems with loading or washes?
Scanning chip
- How many times did you scan?
- Any problems setting PMT gain on lasers so red to green ratio
was ~1.0?
Image processing
- Did you get spots?
- How were the overall intensities across the slide?
- Were there problem areas on the slide (high background, red or green smear, missing spots, etc.)?
Data analysis
- How did you select spots?
- What are your candidate genes for further study?
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Total score:
Copyright, Acknowledgements,
and Intended Use
Created by B. Beason (bbeason@rice.edu),
Rice University, 6 June 2002
Updated 18 July 2006