ADA Colorimetric Activity Assay

The chemical reactions occurring in this enzyme coupled system are shown below:

ADA = adenosine deaminase
PNP = purine nucleoside phosphorylase (EC (bacterial, specific for inosine)
XO = xanthine oxidase (xanthine:oxygen oxidoreductase; EC I: from buttermilk)
PMS = phenazine methosulfate
NBT = nitroblue tetrazolium chloride

Return to Day 6 Procedures.

Copyright, Acknowledgements, and Intended Use
Created by B. Beason (bbeason@rice.edu), Rice University, 16 June 1999
Updated 30 May 2006