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ADA Colorimetric Activity Assay


The chemical reactions occurring in this enzyme coupled system are shown below:


ADA = adenosine deaminase
PNP = purine nucleoside phosphorylase (EC 2.4.2.1) (bacterial, specific for inosine)
XO = xanthine oxidase (xanthine:oxygen oxidoreductase; EC 1.1.3.22)(Grade I: from buttermilk)
PMS = phenazine methosulfate
NBT = nitroblue tetrazolium chloride



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Copyright, Acknowledgements, and Intended Use
Created by B. Beason (bbeason@rice.edu), Rice University, 16 June 1999
Updated 30 May 2006